Biochemical Test and Identification of Citrobacter freundii

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­Basic Characteristics

Properties (Citrobacter freundii)

Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gas from glucose Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Positive (+ve)
H2S Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Facultative Anaerobes
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Negative (-ve)
Urease Variable
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Positive (+ve)
Arabitol Negative (-ve)
Cellobiose Positive (+ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Glutamate Positive (+ve)
Glycerol Positive (+ve)
Glycogen Positive (+ve)
Inositol Negative (-ve)
Lactose Positive (+ve)
Lactulose Positive (+ve)
Malonate Negative (-ve)
Maltitole Negative (-ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Positive (+ve)
Mucate Positive (+ve)
MyoInositol Positive (+ve)
Palatinose Negative (-ve)
Raffinose Variable
Rhamnose Positive (+ve)
Salicin Negative (-ve)
Sorbitol Positive (+ve)
Sorbose Positive (+ve)
Sucrose Positive (+ve)
Tartrate Negative (-ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Acetate Utilization Positive (+ve)
Aesculine Hydrolysis Negative (-ve)
Arginine Dehydrolase Variable
Esculin Hydrolysis Negative (-ve)
Lipase Negative (-ve)
Lysine decarboxylase Negative (-ve)
ONPG (β-galactosidase) Positive (+ve)
Ornithine decarboxylase Negative (-ve)
Phenylalanine deaminase Negative (-ve)
Tryptophan deaminase Variable
Tyrosine hydrolysis Positive (+ve)

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