Negative Staining- Principle, Reagents, Procedure and Result

Result of Negative Staning

The main purpose of Negative staining is to study the morphological shape, size and arrangement of the bacteria cells that is difficult to stain. eg: Spirilla. It can also be used to stain cells that are too delicate to be heat-fixed. It is also used to prepare biological … Read more

Endospore Staining- Principle, Reagents, Procedure and Result

Procedure of Endospore Staining

In 1922, Dorner published a method for staining endospores. Shaeffer and Fulton modified Dorner’s method in 1933 to make the process faster The endospore stain is a differential stain which selectively stains bacterial endospores. The main purpose of endospore staining is to differentiate bacterial spores from … Read more

Capsule Staining- Principle, Reagents, Procedure and Result

Result of Capsule Staining

The main purpose of capsule stain is to distinguish capsular material from the bacterial cell. A capsule is a gelatinous outer layer secreted by bacterial cell and that surrounds and adheres to the cell wall. Most capsules are composed of polysaccharides, but some are composed … Read more

Gram Staining: Principle, Procedure, Interpretation, Examples and Animation

Gram Staining Interpretation

Gram Staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by Danish Bacteriologist Hans Christian Gram in 1884. This test differentiate the bacteria into Gram Positive and Gram Negative Bacteria, which helps in the classification and differentiations of microorganisms. … Read more