Nutrient Agar is a general purpose, nutrient medium used for the cultivation of microbes supporting growth of a wide range of non-fastidious organisms. Nutrient agar is popular because it can grow a variety of types of bacteria and fungi, and contains many nutrients needed for the bacterial growth.
Composition of Nutrient Agar
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0.5% Peptone
It is an enzymatic digest of animal protein. Peptone is the principal source of organic nitrogen for the growing bacteria.
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0.3% beef extract/yeast extract
It is the water-soluble substances which aid in bacterial growth, such as vitamins, carbohydrates, organic nitrogen compounds and salts.
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1.5% agar
It is the solidifying agent.
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0.5% NaCl
The presence of sodium chloride in nutrient agar maintains a salt concentration in the medium that is similar to the cytoplasm of the microorganisms.
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Distilled water
Water is essential for the growth of and reproduction of micro-organisms and also provides the medium through which various nutrients can be transported.
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pH is adjusted to neutral (7.4) at 25 °C.
Preparation of Nutrient Agar
1. Suspend 28 g of nutrient agar powder in 1 litre of distilled water.
2. Heat this mixture while stirring to fully dissolve all components.
3. Autoclave the dissolved mixture at 121 degrees Celsius for 15 minutes.
4. Once the nutrient agar has been autoclaved, allow it to cool but not solidify.
5. Pour nutrient agar into each plate and leave plates on the sterile surface until the agar has solidified.
6. Replace the lid of each Petri dish and store the plates in a refrigerator.
Uses of Nutrients Agar
1. It is frequently used for isolation and purification of cultures.
2. It can also be used as a means for producing the bacterial lawns needed for antibiotic sensitivity tests. In actuality, antibiotic sensitivity testing is typically performed on media specially formulated for that purpose.
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Source: Wikipedia
Source: CHARLES UNIVERSITY
That % mean for 1000ml or 1l you need that particular amount of any substance,like peptone for Nam that is 5gm for 1000ml . Now if you have to prepare low amount of medium then you have to reduce the %. Here you mentioned for 1l media you have to add 1% malt extract for 500lm media it’ll be .5% of malt extract.. That’s how it works
Could you please tell me what exactly we use nutrient agar for
Thanks
Minly it is used for bacterial culture growth.
Show the composition mode preparation and types of organism cultured media
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Two types ke bacteria gram positive and gram negative
Agar are come from huge agar plant know as kelps .are cooked with dilute acid and filtered the acidic liquid is neutralized and cooled so that agal gel is obtained
what are the characteristics of a high quality agar for bacterial growth????
For 1 L media we need 0.4% yeast extract, 1% malt extract, and 0.4% glucose.
My question is: What does % mean?
Thanks.
To prepare 1 L media we need yeast extract 0.4%. malt extract 1%, and glucose 0.4%.
My question is: what does % mean?
Thanks.
0.4 % of 1000 ml media is 0.48*1000/100= 4 gm/ Liter of the solute.
1% of 1000 ml media is 1*1000/100= 10 gm/Lt.
Are you kidding Ritesh Sharma
0.48*1000/100=4gm….how dude…
It’ll be 0.48*1000/100=4.8gm
What about adding he chemical formula?
What about nutrient broth ? Composition ?
I dont know about that but you can add a broad spectrum antibiotic to avoid griwth of bacteria in your plates
Whats the basic defferences between all culture media
And which culture media would use for which????
Sir I have used nutrient agar for fungus culture…. Before i use PDA for culture it was gud…. But i found lots of bacterial contamination in my Nutrient agar culture…. Does the composition of beef extract hampers the groeth of fungus and favours bacteria instead.
how microbes get water content while they are cultured on the nutrient agar plates
What is Basal media????
peptone water, Nutrient broth, and nutrient agar. non enriched general purpose media.
What is Basal media
It media that supports the growth of bacteria but do not need nutrients
can this basal media use for bacterial survivalence.
how to subculture B.pulimnus
Hello I have a question. If a leg wound is cultured and it is put on a nutrient agar, Selective agar and Differential Agar what are expected to show?
What is the principle or action of nutrient media?
Hi ! I streaked out from sweet potato steep water and I had both red colonies and white colonies that turned nutrient agar kind of green. Please what kind of organisms are these . They areboth staining gram positive
yes both are gram positive
E.coli
Nutrient agar favored 4 Non fastidious bacteria
Blood agar used to detect bacteria that produce enzymes to break apart the Red blood cells
Mac conkey agar favored 4 gram – bacteria
sabouraud agar favoured 4 fungi
lowenstein jensen agar favored 4 mycobacterium tuberculosis
salmonella shigella agar favored in the isolation of salmonella&shigella
1.Basal media
2.Enriched media
3.Selective media
4.Enrichment media
5.Differential media
the compositon and funtions were helpful. but i dont really get the beef extract could you please explain more. you did a nice job. my name is Anie, 19yr old microbiology student, i’m currently in my 3rd year in Uni
among the types of medias we have can somebody help me and tell me the kind of micro organism to which each media favour the growth
eg nutrient agar favour the growth of …..
which dye is used for nutrient agar
what is the color of nutrient agar?
go close to d practical,and the Petri dish you get to see the colour after inoculation and culture
cremish brown.
Sir/Madam,
Could u tell me what are the microbial techniques? Just list put techniques name so that i could learn it from basics.
Thank you
Could u tell me what are the microbial techniques? Just list out the techniques name so that i could learn it from basics.
goodnight
I wonder about questions such as Pseudomonas aeruginosa colonies on nutrient agar?