Mannitol Salt Agar for the isolation of Staphylococcus aureus

Mannitol Salt Agar for the isolation of Staphylococcus aureus

Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. It is a selective medium prepared according to the recommendations of Chapman for the …

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Bird Seed Agar for the isolation of Cryptococcus neoformans

Bird Seed Agar for the isolation of Cryptococcus neoformans

Bird Seed Agar is a solid medium used for selective and differential isolation of Cryptococcus neoformans from clinical specimens. Cryptococcus neoformans is an encapsulated yeast that produces the enzyme phenoloxidase, an enzyme necessary in melanin synthesis. The brown pigmented colonies of Cryptococcus neoformans were observed by Staib in 1962 when he grew cultures of the yeast …

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Salmonella Shigella (SS) Agar- Composition, Principle, Uses, Preparation and Result Interpretation

Result Interpretation on Salmonella Shigella Agar

Salmonella Shigella (SS) Agar is moderately selective and differential medium for the isolation, cultivation and differentiation of Salmonella spp. and some strains of Shigella spp. SS Agar is a modification of the Desoxycholate Citrate Agar. It is recommended for testing clinical specimens and food testing for the presence of Salmonella spp. and some Shigella spp. …

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Starch Casein Agar (SCA) – Composition, Principle, Uses, Preparation and Result Interpretation

Actinomycetes on Starch Casein Agar (SCA)

Starch Casein Agar (SCA) is used for the detection of saccharolytic marine bacteria and mostly actinomycetes. Actinomycetes are a fungi-like bacteria forming long filaments that stretch through the soil. They are large group of gram positive filamentous and/ or branching bacilli. Actinomycetes have been isolated from terrestrial sources, although the first report of mycelium forming …

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List of culture media used in microbiology with their uses

Media Microbiology

Media Uses ·   Alkaline Peptone Water Enrichment media for Vibrio cholerae ·   Alkaline Salt Transport Medium ·   Taurocholate Peptone Transport Medium Transport media for diarrheal diseases suspected of being caused by V. cholerae ·   Anaerobic Media Liquid media by addition of ·     Glucose (0.5 % to 1 %) ·     Ascorbic Acid (0.1 %) ·     Cysteine …

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Selenite F Broth- Composition, Principle, Uses, Preparation and Result Interpretation

Selenite F Broth- Composition, Principle, Uses, Preparation and Result Interpretation

Selenite F Broth is the medium used for the selective enrichment of Salmonella spp from both clinical and food samples. It is a buffered Lactose Peptone Broth to which Sodium Biselenite is added as the selective agent. Selenite-F Broth was devised by Leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as …

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Simmons Citrate Agar- Composition, Principle, Uses, Preparation and Result Interpretation

Result Interpretation on Simmons Citrate Agar

Simmons Citrate Agar is an agar medium used for the differentiation of Enterobacteriaceae based on the utilization of citrate as the sole source of carbon.  In the early 1920s, Koser developed a liquid medium formulation for the differentiation of fecal coliforms from the coliform group. Simmons later modified this formulation to produce a solid medium …

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Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar- Composition, Principle, Uses, Preparation and Colony Morphology

Colony Morphology on TCBS Agar

TCBS Agar is used for the selective isolation of cholera vibrios and Vibrio parahaemolyticus from a variety of clinical and nonclinical specimens. Vibrio species are most widely recognized for their role in human intestinal infections, and cholera and V. parahaemolyticus diarrhea are important worldwide. All pathogenic Vibrio spp., except Vibrio hollisae, will grow on TCBS Agar. The original formula developed by Nakanishi was subsequently …

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Lowenstein-Jensen (LJ) Medium- Composition, Principle, Uses, Preparation and Colony Morphology

Colony Morphology on LJ Medium

Lowenstein-Jensen (LJ) is the selective medium which is used for the cultivation and isolation of Mycobacterium species. It was developed by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria. The present formulation, a glycerated egg-based medium, is based upon Jensen’s modification. Jensen’s version eliminates congo red and uses a moderate concentration of malachite green …

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MacConkey Agar- Composition, Principle, Uses, Preparation and Colony Morphology

Colony Morphology on MacConkey Agar

MacConkey agar (MAC) was the first solid differential media to be formulated which was developed at 20th century by Alfred Theodore MacConkey. MacConkey agar is a selective and differential media used for the isolation and differentiation of non-fastidious gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas. Composition of MacConkey Agar Ingredients Amount Peptone (Pancreatic …

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Sabouraud Dextrose Agar (SDA) – Composition, Principle, Uses, Preparation and Colony Morphology

Colony Morphology on SDA

Sabouraud Dextrose Agar (SDA) is used for the isolation, cultivation, and maintenance of non-pathogenic and pathogenic species of fungi and yeasts. SDA was formulated by Sabouraud in 1892 for culturing dermatophytes. The pH is adjusted to approximately 5.6 in order to enhance the growth of fungi, especially dermatophytes, and to slightly inhibit bacterial growth in clinical specimens. Composition …

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Cetrimide Agar- Composition, Principle, Uses, Preparation and Colony Morphology

Colony Morphology on Cetrimide Agar

Cetrimide Agar is a selective and differential medium used for the isolation and identification of Pseudomonas aeruginosa from clinical and non-clinical specimens.  Cetrimide is the selective agent and inhibits most bacteria by acting as a detergent ( Cetyltrimethylammonium bromide, a quaternary ammonium, cationic detergent). It is also known as Pseudomonas Cetrimide Agar or Pseudosel Agar. Composition of Cetrimide Agar Ingredients In …

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Mueller Hinton Agar (MHA) – Composition, Principle, Uses and Preparation

Mueller Hinton Agar (MHA) – Composition, Principle, Uses and Preparation

Mueller and Hinton developed Mueller Hinton Agar (MHA) in 1941 for the isolation of pathogenic Neisseria species. Nowadays, it is more commonly used for the routine susceptibility testing of non-fastidious microorganism by the Kirby-Bauer disk diffusion technique. Five percent sheep blood and nicotinamide adenine dinucleotide may also be added when susceptibility testing is done on Streptococcus species. This …

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Xylose Lysine Deoxycholate (XLD) Agar- Principle, Uses, Composition, Preparation and Colony Characteristics

Shigella and Salmonella Colonies on XLD Agar

Xylose Lysine Deoxycholate (XLD) Agar is a selective medium for the isolation of Salmonella and Shigella spp from clinical specimens and food samples. XLD Agar was originally formulated by Taylor for the isolation and identification of Shigella from stool specimens.  The pathogens are differentiated not only from the non-pathogenic lactose fermenters but also from many non-pathogens which do not ferment lactose …

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Potato Dextrose Agar (PDA)- Principle, Uses, Composition, Procedure and Colony Characteristics

Colony Characteristics on Potato Dextrose Agar (PDA)

Potato Dextrose Agar (PDA) is used for the cultivation of fungi. Potato Dextrose Agar (PDA) is a general purpose medium for yeasts and molds that can be supplemented with acid or antibiotics to inhibit bacterial growth. It is recommended for plate count methods for foods, dairy products and testing cosmetics. PDA can be used for growing clinically significant yeast and …

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