Mannitol Salt Agar for the isolation of Staphylococcus aureus

Mannitol Salt Agar for the isolation of Staphylococcus aureus

Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. It is a selective … Read more

Bird Seed Agar for the isolation of Cryptococcus neoformans

Bird Seed Agar for the isolation of Cryptococcus neoformans

Bird Seed Agar is a solid medium used for selective and differential isolation of Cryptococcus neoformans from clinical specimens. Cryptococcus neoformans is an encapsulated yeast that produces the enzyme phenoloxidase, an enzyme necessary in melanin synthesis. The brown pigmented colonies of Cryptococcus neoformans were observed by … Read more

List of culture media used in microbiology with their uses

Media Microbiology

Media Uses · Alkaline Peptone Water Enrichment media for Vibrio cholerae · Alkaline Salt Transport Medium · Taurocholate Peptone Transport Medium Transport media for diarrheal diseases suspected of being caused by V. cholerae · Anaerobic Media Liquid media by addition of · Glucose (0.5 % … Read more

Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar- Composition, Principle, Uses, Preparation and Colony Morphology

Colony Morphology on TCBS Agar

TCBS Agar is used for the selective isolation of cholera vibrios and Vibrio parahaemolyticus from a variety of clinical and nonclinical specimens. Vibrio species are most widely recognized for their role in human intestinal infections, and cholera and V. parahaemolyticus diarrhea are important worldwide. All pathogenic Vibrio spp., except Vibrio hollisae, will grow … Read more

Cetrimide Agar- Composition, Principle, Uses, Preparation and Colony Morphology

Colony Morphology on Cetrimide Agar

Cetrimide Agar is a selective and differential medium used for the isolation and identification of Pseudomonas aeruginosa from clinical and non-clinical specimens.  Cetrimide is the selective agent and inhibits most bacteria by acting as a detergent ( Cetyltrimethylammonium bromide, a quaternary ammonium, cationic detergent). It is also known as … Read more

Mueller Hinton Agar (MHA) – Composition, Principle, Uses and Preparation

Mueller Hinton Agar (MHA) – Composition, Principle, Uses and Preparation

Mueller and Hinton developed Mueller Hinton Agar (MHA) in 1941 for the isolation of pathogenic Neisseria species. Nowadays, it is more commonly used for the routine susceptibility testing of non-fastidious microorganism by the Kirby-Bauer disk diffusion technique. Five percent sheep blood and nicotinamide adenine dinucleotide may also … Read more

Blood Agar- Composition, Preparation, Uses and Pictures

Haemolysis

Blood Agar (BA) are enriched medium used to culture those bacteria or microbes that do not grow easily. Such bacteria are called “fastidious” as they demand a special, enriched nutritional environment as compared to the routine bacteria. Blood Agar is used to grow a wide … Read more

Nutrient Agar: Composition, Preparation and Uses

Four nutrient agar plates growing colonies of common Gram negative bacteria.

Nutrient Agar is a general purpose, nutrient medium used for the cultivation of microbes supporting growth of a wide range of non-fastidious organisms. Nutrient agar is popular because it can grow a variety of types of bacteria and fungi, and contains many nutrients needed for the bacterial growth. … Read more