Biochemical Test and Identification of Shigella flexneri

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­Basic Characteristics Properties (Shigella flexneri)
Catalase Positive (+ve)
Citrate Negative (-ve)
Gas Positive (+ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Negative (-ve)
H2S Negative (-ve)
Indole Variable
Motility Non-Motile
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Positive (+ve)
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Non-Sporing
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)
Fermentation of
Adonitol Negative (-ve)
Arabinose Variable
Cellobiose Negative (-ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Glycerol Negative (-ve)
Lactose Negative (-ve)
Malonate Negative (-ve)
Maltose Variable
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Variable
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Raffinose Variable
Rhamnose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Variable
Sucrose Negative (-ve)
Tartrate Variable
Trehalose Positive (+ve)
Xylose Negative (-ve)
Enzymatic Reactions
Acetate Utilization Negative (-ve)
Aesculine Hydrolysis Negative (-ve)
Arginine Dehydrolase Negative (-ve)
Lipase Negative (-ve)
Lysine Negative (-ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Phenylalanine Deaminase Negative (-ve)

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