Biochemical Test and Identification of Clostridium difficile

­Basic Characteristics

Properties (Clostridium difficile)

Capsule Capsulated
Catalase Negative (-ve)
Flagella Flagellated
Gelatin Hydrolysis Positive (+ve)
Gram Staining Positive (+ve)
H2S Positive (+ve)
Hemolysis Negative (-ve)
Indole Negative (-ve)
Motility Motile
Nitrate Reduction Negative (-ve)
OF (Oxidative-Fermentative) Anaerobic
Oxidase Negative (-ve)
Shape Rods
Spore Positive (+ve)
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Arabinose Negative (-ve)
Cellobiose Positive (+ve)
Dulcitol Variable
Fructose Positive (+ve)
Galactose Negative (-ve)
Glucose Positive (+ve)
Glycerol Negative (-ve)
Glycogen Negative (-ve)
Inositol Negative (-ve)
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Ribose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Trehalose Negative (-ve)
Xylose Negative (-ve)

Enzymatic Reactions

Casein Hydrolysis Negative (-ve)
Esculin Hydrolysis Positive (+ve)
Lecithinase Negative (-ve)
Lipase Negative (-ve)
Tryptophan Deaminase Negative (-ve)

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