Biochemical Test and Identification of Bacillus subtilis

­Basic Characteristics

Properties (Bacillus subtilis)

CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaFlagellated
GasNegative (-ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingGram Positive (+ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Negative (-ve)
Nitrate ReductionPositive (+ve)
OxidaseVariable
PigmentNegative (-ve)
ShapeRods
SporePositive (+ve)
UreaseNegative (-ve)
VP (Voges Proskauer)Positive (+ve)

Fermentation of

AdonitolNegative (-ve)
ArabinosePositive (+ve)
ArabitolNegative (-ve)
CellobiosePositive (+ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
FructosePositive (+ve)
GalactoseVariable
GlucosePositive (+ve)
GlycerolPositive (+ve)
GlycogenPositive (+ve)
InositolPositive (+ve)
InulinVariable
LactoseVariable
MaltosePositive (+ve)
MannitolPositive (+ve)
MannosePositive (+ve)
MelibioseVariable
RaffinosePositive (+ve)
RhamnoseNegative (-ve)
RibosePositive (+ve)
SalicinPositive (+ve)
SorbitolPositive (+ve)
StarchPositive (+ve)
SucrosePositive (+ve)
TrehalosePositive (+ve)
XylosePositive (+ve)

Enzymatic Reactions

Arginine DehydrolaseNegative (-ve)
Casein HydrolysisPositive (+ve)
Esculin HydrolysisPositive (+ve)
LecithinaseNegative (-ve)
LysineNegative (-ve)
Ornithine DecarboxylaseNegative (-ve)
Phenylalanine DeaminaseNegative (-ve)
Tyrosine HydrolysisNegative (-ve)

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