|Illuminating source is the Light.
|Illuminating source is the beam of electrons.
|Specimen preparation takes usually few minutes to hours.
|Specimen preparation takes usually takes few days.
|Live or Dead specimen may be seen.
|Only Dead or Dried specimens are seen.
|Condenser, Objective and eye piece lenses are made up of glasses.
|All lenses are electromagnetic.
|It has low resolving power (0.25µm to 0.3µm).
|It has high resolving power (0.001µm), about 250 times higher than light microscope.
|It has a magnification of of 500X to 1500X.
|It has a magnification of 100,000X to 300,000X.
|The object is 5µm or thicker.
|The object is 0.1µm or thinner.
|Image is Colored.
|Image is Black and White.
|Vacuum is not required.
|Vacuum is essential for its operation.
|There is no need of high voltage electricity.
|High voltage electric current is required (50,000 Volts and above).
|There is no cooling system.
|It has a cooling system to take out heat generated by high electric current.
|Filament is not used.
|Tungsten filament is used to produce electrons.
|Radiation risk is absent.
|There is risk of radiation leakage.
|Specimen is stained by colored dyes.
|Specimen is coated with heavy metals in order to reflect electrons.
|Image is seen by eyes through ocular lens.
|Image is received in Zinc Sulphate Fluorescent Screen or Photographic Plate.
|It is used for the study of detailed gross internal structure.
|It is used in the study of external surface, ultra structure of cell and very small organisms.