Biochemical Test and Identification of Proteus mirabilis

­Basic Characteristics

Properties ( Proteus mirabilis )

Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Positive (+ve)
Flagella Positive (+ve)
Gas from Glucose Positive (+ve)
Gelatin Hydrolysis Positive (+ve)
Gram Staining Negative (-ve)
Growth in KCN Positive (+ve)
H2S Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
MR (Methyl Red) Positive (+ve)
Nitrate Reduction Positive (+ve)
OF (Oxidative-Fermentative) Facultative anaerobes
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rods
Spore Negative (-ve)
Urease Positive (+ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Arabitol Negative (-ve)
Cellobiose Negative (-ve)
DNase Variable
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Glucose Positive (+ve)
Glycerol Positive (+ve)
Inositol Negative (-ve)
Lactose Negative (-ve)
Malonate Negative (-ve)
Maltose Negative (-ve)
Mannitol Negative (-ve)
Mannose Negative (-ve)
Melibiose Negative (-ve)
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Negative (-ve)
Starch Negative (-ve)
Sucrose Negative (-ve)
Tartrate Positive (+ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Acetate Utilization Negative (-ve)
Aesculine Hydrolysis Negative (-ve)
Casein Hydrolysis Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Lipase Positive (+ve)
Lysine decarboxylases Negative (-ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Positive (+ve)
Pectate Degradation Negative (-ve)
Phenylalanine Deaminase Positive (+ve)
Tryptophan Deaminase Negative (-ve)

5 thoughts on “Biochemical Test and Identification of Proteus mirabilis”

    • Loefler’s serum slant or Tindales agar are selective media for cornye. Cornye has a particular look on the Tinsdale agar. Then you’ll do your regular gram stains (Gram Positive bacilli, sometimes with club shape), catalase (positive), urease test (negative), growth is not supported on a MacConkey agar. Albert stain for confirmation to show green tail and reddish metachromatic granules. Carbohydrate Fermentation test: Maltose- Negative , Dextrose-Positive, Starch-Positive, Sucrose-Negative. The positive results for the starch and the negative results for the sucrose show the presence of a species of Corynebacterium diphtheriae (gravis).

    • Nutrient agar is always a safe choice. MacConkey is usually fairly decent, especially if you want to test the lactose fermentation thing. Finally, blood agar is always really cool for p. mirabilis, because the organism has a tendency to swarm across the agar (because of it’s high motility), so that’s cool to see.

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