Biochemical Test and Identification of Proteus mirabilis

Basic CharacteristicsProperties (Proteus mirabilis)
CapsuleNegative (-ve)
CatalasePositive (+ve)
CitratePositive (+ve)
FlagellaPositive (+ve)
Gas from GlucosePositive (+ve)
Gelatin HydrolysisPositive (+ve)
Gram StainingNegative (-ve)
Growth in KCNPositive (+ve)
H2SPositive (+ve)
IndoleNegative (-ve)
MotilityPositive (+ve)
MR (Methyl Red)Positive (+ve)
Nitrate ReductionPositive (+ve)
OF (Oxidative-Fermentative)Facultative anaerobes
OxidaseNegative (-ve)
PigmentNegative (-ve)
SporeNegative (-ve)
UreasePositive (+ve)
VP (Voges Proskauer)Negative (-ve)
Fermentation of
AdonitolNegative (-ve)
ArabinoseNegative (-ve)
ArabitolNegative (-ve)
CellobioseNegative (-ve)
DulcitolNegative (-ve)
ErythritolNegative (-ve)
GlucosePositive (+ve)
GlycerolPositive (+ve)
InositolNegative (-ve)
LactoseNegative (-ve)
MalonateNegative (-ve)
MaltoseNegative (-ve)
MannitolNegative (-ve)
MannoseNegative (-ve)
MelibioseNegative (-ve)
MucateNegative (-ve)
MyoInositolNegative (-ve)
RaffinoseNegative (-ve)
RhamnoseNegative (-ve)
SalicinNegative (-ve)
SorbitolNegative (-ve)
StarchNegative (-ve)
SucroseNegative (-ve)
TartratePositive (+ve)
TrehalosePositive (+ve)
XylosePositive (+ve)
Enzymatic Reactions
Acetate UtilizationNegative (-ve)
Aesculine HydrolysisNegative (-ve)
Casein HydrolysisNegative (-ve)
Esculin HydrolysisNegative (-ve)
LipasePositive (+ve)
Lysine decarboxylasesNegative (-ve)
ONPG (β-galactosidase)Negative (-ve)
Ornithine DecarboxylasePositive (+ve)
Pectate DegradationNegative (-ve)
Phenylalanine DeaminasePositive (+ve)
Tryptophan DeaminaseNegative (-ve)

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9 thoughts on “Biochemical Test and Identification of Proteus mirabilis”

    • first and foremost the most identifications of test for proteus spp by urease test ,PPA
      The socond biochemical test indole the are defrenciate to p. Mirabilis from p vulgaris. If indole positve_ P.vllulgaris if negative – P.mirabilis

    • P.vulgaris is urease + within 3 hours, PPA +, methyl red +, cirrate +, lactose -, maltose +, sucrose +, ornithine decarboxylase -, Gram -,ve rod and actively motile.

    • Loefler’s serum slant or Tindales agar are selective media for cornye. Cornye has a particular look on the Tinsdale agar. Then you’ll do your regular gram stains (Gram Positive bacilli, sometimes with club shape), catalase (positive), urease test (negative), growth is not supported on a MacConkey agar. Albert stain for confirmation to show green tail and reddish metachromatic granules. Carbohydrate Fermentation test: Maltose- Negative , Dextrose-Positive, Starch-Positive, Sucrose-Negative. The positive results for the starch and the negative results for the sucrose show the presence of a species of Corynebacterium diphtheriae (gravis).

    • Nutrient agar is always a safe choice. MacConkey is usually fairly decent, especially if you want to test the lactose fermentation thing. Finally, blood agar is always really cool for p. mirabilis, because the organism has a tendency to swarm across the agar (because of it’s high motility), so that’s cool to see.


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