Biochemical Test and Identification of E. coli

Characteristics E. coli
Gram Staining Negative
Shape (Cocci/Diplococci/Rods) Rods
Motility (Motile / Non-Motile) Motile
Capsule (Capsulated/Non-Capsulated) Variable
Spore (Sporing/Non-Sporing) Non-Sporing
Flagella (Flagellated/Non-Flagellated) Flagellated
Catalase Positive (+ve)
Oxidase Negative (-ve)
MR Positive (+ve)
VP Negative (-ve)
OF (Oxidative/Fermentative) Fermentative
Indole Positive (+ve)
Citrate Negative (-ve)
Urease Negative (-ve)
Nitrate Reduction Positive (+ve)
H2S Negative (-ve)
Gas Positive (+ve)
PYR
CAMP
Gelatin Negative (-ve)
Niacin
Coagulase
Hemolysis (Alfa/Beta/Gamma) Some Strains shows Hemolysis
Nagler
String Test
Pigment
Bile Solubility
Fermentation of
Arabinose Positive (+ve)
DNase Negative (-ve)
Fructose
Glucose Positive (+ve)
Hippurate
Inositol Negative (+ve)
Inulin
Lactose Positive (+ve)
Maltose
Mannitol Positive (+ve)
Mannose
MyoInositol
Raffinose
Ribose
Sorbitol Positive (+ve)
Sucrose Variable
Enzymatic Reactions
Acetate Utilization Positive (+ve)
Acid Phosphatase
Alkaline Phosphatase
Amidase
Arginine dehydrolase Variable
Arylsulphatase
Beta Lactamase
Hyalurodinase
Lecithinase
Lipase
Lysine Positive (+ve)
Neuraminidase
ONPG Test Positive (+ve)
Ornithine decarboxylase Variable
Phenylalanine deaminase Negative (-ve)
Perooxidase
Tellurite
Tributyrin
Differential Antibiotic
Novobiocin
Bacitracin
Optochin
Streptomysin

7 thoughts on “Biochemical Test and Identification of E. coli”

  1. Mannitol is an alcohol-based compound, the mannitol solution does not contain salt in it. What you guys are arguing about was probably Mannitol salt agar.

  2. Can you please help me, with some test who detected the
    1. Candida spp and
    2. Gardnerella vaginalis?

    in form +;- in who react and who nonreact. In the way of escherichia coli test.

    Honestly,
    Annais

  3. Our lake has been tested for E. Coli and the results were given as such: 1: >80. 2: 5. 3: 5. and 4: 55. I assume they did 5 separate tests but did not explain if these are high or low and whether our is contaminated to the point that we cannot swim in or uuse its water to take showers or let the dogs in it since they drink the water. Can you help us figure this out.

  4. Mnnitol culture E.coli may be a new strain of E.coli because Mannitol have 9-11% NACL and every type of bacteria could not tolrate such amount of salt concentration.
    It need to identify on Molecular basis

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