Biochemical Test and Identification of Pseudomonas aeruginosa

Characteristics

Pseudomonas aeruginosa

Gram StainingNegative
Shape (Cocci/Diplococci/Rods)Rods
Motility (Motile / Non-Motile)Motile (Unipolar)
Capsule (Capsulated/Non-Capsulated)Non-Capsulated
Spore (Sporing/Non-Sporing)Non-Sporing
Flagella (Flagellated/Non-Flagellated)Single Flagella
CatalasePositive (+ve)
OxidasePositive (+ve)
MRNegative (-ve)
VPNegative (-ve)
OF (Oxidative/Fermentative)Oxidative
IndoleNegative (-ve)
CitratePositive (+ve)
UreaseNegative (-ve)
Nitrate ReductionPositive (+ve)
H2SNegative (-ve)
GasPositive (+ve)- From Nitrate
PYR
CAMP
Gelatin HydrolysisPositive (+ve)
Niacin
CoagulaseNegative (-ve)
Hemolysis (Alfa/Beta/Gamma)
Nagler
String Test
PigmentPositive (+ve)
Bile Solubility
Cetrimide Test Positive (+ve)

Fermentation of

Arabinose
DNase
Fructose
GlucoseNegative (-ve)
Hippurate
Inositol
InulinNegative (-ve)
LactoseNegative (-ve)
MaltoseNegative (-ve)
MannitolPositive (+ve)
Mannose
MyoInositol
Raffinose
Ribose
SorbitolNegative (-ve)
SucroseNegative (-ve)

Enzymatic Reactions

Acetate Utilization
Acetoin Production
Acid Phosphatase
Alkaline Phosphatase
Amidase
Arginine dehydrolasePositive (+ve)
Arylsulphatase
Beta Lactamase
Hyalurodinase
Lecithinase
LipasePositive (+ve)
LysineNegative (-ve)
Neuraminidase
ONPG Test
Ornithine decarboxylaseNegative (-ve)
Phenylalanine deaminase
Perooxidase
Tellurite
Tributyrin

14 thoughts on “Biochemical Test and Identification of Pseudomonas aeruginosa”

  1. I got gram negative short rods,catalase +, oxidase -, H2s -,methyl red-, voges proskaeur-, lactose -, maltose -e, inositol -, sucrose-, Alk/Alk no Gas, Argenine- , Lysin-, Ornitine- , tolerate salt 4%, SIM in 30c+, SIM in 37c -, growing on Maconky, could you help me, to identity the organism species.
    Thanks in advance

    Reply
  2. I got gram negative short rods,catalase +ve,coagulase -ve,citrate+ve,non motile,insole/H2s -ve,oxidase +ve,methyl red+ve,voges proskaeur-ve,glucose ferm.+ve,lactose -ve,maltose+ve,mannitol -ve,sucrose+ve,blue green pigment, could you help me, to identity the organism species.

    Reply
  3. sir,
    I am doing my project work on Isolation, Characterization and Bioefficacy studies of Pseudomonas Spp.
    I got the HI MEDIA Biochemical test results as follows:
    Citrate test +ve, Lysine +ve, Ornithine +ve, Urease +ve, Phenylalanine deamination -ve, Nitrate reduction -ve, H2S Production +ve, Glucose -ve, Adonitol-ve, Lactose -ve, Arabinose +ve, Sorbitol-ve.
    I am unable to identify which Pseudomonas Spp. it is.
    Can you please help me in identifying the Pseudomonas Spp.

    Reply
  4. I’m taking micro now, and my professor says that both tests can’t share the same result. Why are the MV and PR tests both negative? I have an unknown with green pigment, and many matching results on this list. Both my MV and PR tests were negative. What should be my reasoning in my report? thank you

    Reply
    • probably your professor was talking about Enterobacteriaceae (negative rod bacteria). Pseudomonas sp is negative baci but Non-Enterobacteriaceae.

      Reply
  5. what is the selective media ? which media is used for confirmation of Pseudomonas aeruginosa.
    What is the colony charecteristics or indicative properties on the selective and confirmatory agar ?

    Reply
  6. Thank you Sagar Aryal sir..this is exactly the information I needed (infact this is the best information that i have found so far)..I too am a student of B.Sc. MB at St.xaviers ………extremely helpful information….thanks again

    Reply

Leave a Comment