Biochemical Test and Identification of Pseudomonas aeruginosa

Characteristics

Pseudomonas aeruginosa

Gram Staining Negative
Shape (Cocci/Diplococci/Rods) Rods
Motility (Motile / Non-Motile) Motile (Unipolar)
Capsule (Capsulated/Non-Capsulated) Non-Capsulated
Spore (Sporing/Non-Sporing) Non-Sporing
Flagella (Flagellated/Non-Flagellated) Single Flagella
Catalase Positive (+ve)
Oxidase Positive (+ve)
MR Negative (-ve)
VP Negative (-ve)
OF (Oxidative/Fermentative) Oxidative
Indole Negative (-ve)
Citrate Positive (+ve)
Urease Negative (-ve)
Nitrate Reduction Positive (+ve)
H2S Negative (-ve)
Gas Positive (+ve)- From Nitrate
PYR
CAMP
Gelatin Hydrolysis Positive (+ve)
Niacin
Coagulase Negative (-ve)
Hemolysis (Alfa/Beta/Gamma)
Nagler
String Test
Pigment Positive (+ve)
Bile Solubility
Cetrimide Test Positive (+ve)

Fermentation of

Arabinose
DNase
Fructose
Glucose Negative (-ve)
Hippurate
Inositol
Inulin Negative (-ve)
Lactose Negative (-ve)
Maltose Negative (-ve)
Mannitol Positive (+ve)
Mannose
MyoInositol
Raffinose
Ribose
Sorbitol Negative (-ve)
Sucrose Negative (-ve)

Enzymatic Reactions

Acetate Utilization
Acetoin Production
Acid Phosphatase
Alkaline Phosphatase
Amidase
Arginine dehydrolase Positive (+ve)
Arylsulphatase
Beta Lactamase
Hyalurodinase
Lecithinase
Lipase Positive (+ve)
Lysine Negative (-ve)
Neuraminidase
ONPG Test
Ornithine decarboxylase Negative (-ve)
Phenylalanine deaminase
Perooxidase
Tellurite
Tributyrin

12 thoughts on “Biochemical Test and Identification of Pseudomonas aeruginosa”

  1. sir,
    I am doing my project work on Isolation, Characterization and Bioefficacy studies of Pseudomonas Spp.
    I got the HI MEDIA Biochemical test results as follows:
    Citrate test +ve, Lysine +ve, Ornithine +ve, Urease +ve, Phenylalanine deamination -ve, Nitrate reduction -ve, H2S Production +ve, Glucose -ve, Adonitol-ve, Lactose -ve, Arabinose +ve, Sorbitol-ve.
    I am unable to identify which Pseudomonas Spp. it is.
    Can you please help me in identifying the Pseudomonas Spp.

  2. I’m taking micro now, and my professor says that both tests can’t share the same result. Why are the MV and PR tests both negative? I have an unknown with green pigment, and many matching results on this list. Both my MV and PR tests were negative. What should be my reasoning in my report? thank you

    • probably your professor was talking about Enterobacteriaceae (negative rod bacteria). Pseudomonas sp is negative baci but Non-Enterobacteriaceae.

  3. what is the selective media ? which media is used for confirmation of Pseudomonas aeruginosa.
    What is the colony charecteristics or indicative properties on the selective and confirmatory agar ?

  4. Thank you Sagar Aryal sir..this is exactly the information I needed (infact this is the best information that i have found so far)..I too am a student of B.Sc. MB at St.xaviers ………extremely helpful information….thanks again

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