| Gram Staining | Negative |
| Shape (Cocci/Diplococci/Rods) | Rods |
| Motility (Motile / Non-Motile) | Motile (Unipolar) |
| Capsule (Capsulated/Non-Capsulated) | Non-Capsulated |
| Spore (Sporing/Non-Sporing) | Non-Sporing |
| Flagella (Flagellated/Non-Flagellated) | Single Flagella |
| Catalase | Positive (+ve) |
| Oxidase | Positive (+ve) |
| MR | Negative (-ve) |
| VP | Negative (-ve) |
| OF (Oxidative/Fermentative) | Oxidative |
| Indole | Negative (-ve) |
| Citrate | Positive (+ve) |
| Urease | Negative (-ve) |
| Nitrate Reduction | Positive (+ve) |
| H2S | Negative (-ve) |
| Gas | Positive (+ve)- From Nitrate |
| PYR | – |
| CAMP | – |
| Gelatin Hydrolysis | Positive (+ve) |
| Niacin | – |
| Coagulase | Negative (-ve) |
| Hemolysis (Alfa/Beta/Gamma) | – |
| Nagler | – |
| String Test | – |
| Pigment | Positive (+ve) |
| Bile Solubility | – |
| Cetrimide Test | Positive (+ve) |
| Fermentation of |
| Arabinose | – |
| DNase | – |
| Fructose | – |
| Glucose | Negative (-ve) |
| Hippurate | – |
| Inositol | – |
| Inulin | Negative (-ve) |
| Lactose | Negative (-ve) |
| Maltose | Negative (-ve) |
| Mannitol | Positive (+ve) |
| Mannose | – |
| MyoInositol | – |
| Raffinose | – |
| Ribose | – |
| Sorbitol | Negative (-ve) |
| Sucrose | Negative (-ve) |
| Enzymatic Reactions |
| Acetate Utilization | – |
| Acetoin Production | – |
| Acid Phosphatase | – |
| Alkaline Phosphatase | – |
| Amidase | – |
| Arginine dehydrolase | Positive (+ve) |
| Arylsulphatase | – |
| Beta Lactamase | – |
| Hyalurodinase | – |
| Lecithinase | – |
| Lipase | Positive (+ve) |
| Lysine | Negative (-ve) |
| Neuraminidase | – |
| ONPG Test | – |
| Ornithine decarboxylase | Negative (-ve) |
| Phenylalanine deaminase | – |
| Perooxidase | – |
| Tellurite | – |
| Tributyrin | – |
I got gram negative short rods,catalase +, oxidase -, H2s -,methyl red-, voges proskaeur-, lactose -, maltose -e, inositol -, sucrose-, Alk/Alk no Gas, Argenine- , Lysin-, Ornitine- , tolerate salt 4%, SIM in 30c+, SIM in 37c -, growing on Maconky, could you help me, to identity the organism species.
Thanks in advance
Pseudomonase aeruginosa
I got gram negative short rods,catalase +ve,coagulase -ve,citrate+ve,non motile,insole/H2s -ve,oxidase +ve,methyl red+ve,voges proskaeur-ve,glucose ferm.+ve,lactose -ve,maltose+ve,mannitol -ve,sucrose+ve,blue green pigment, could you help me, to identity the organism species.
Please refer Cowan and Steel manual for the identification of medical bacteria
does anyone know how to successfully treat pseudomonas
sir,
I am doing my project work on Isolation, Characterization and Bioefficacy studies of Pseudomonas Spp.
I got the HI MEDIA Biochemical test results as follows:
Citrate test +ve, Lysine +ve, Ornithine +ve, Urease +ve, Phenylalanine deamination -ve, Nitrate reduction -ve, H2S Production +ve, Glucose -ve, Adonitol-ve, Lactose -ve, Arabinose +ve, Sorbitol-ve.
I am unable to identify which Pseudomonas Spp. it is.
Can you please help me in identifying the Pseudomonas Spp.
You can go for 16S rRNA sequencing for the identification
is that really Pseudomonas aeruginosa is LDC negative????
I’m taking micro now, and my professor says that both tests can’t share the same result. Why are the MV and PR tests both negative? I have an unknown with green pigment, and many matching results on this list. Both my MV and PR tests were negative. What should be my reasoning in my report? thank you
probably your professor was talking about Enterobacteriaceae (negative rod bacteria). Pseudomonas sp is negative baci but Non-Enterobacteriaceae.
its good if you mentions colony morphology..
what is the selective media ? which media is used for confirmation of Pseudomonas aeruginosa.
What is the colony charecteristics or indicative properties on the selective and confirmatory agar ?
Selective media :- centrimide agar
Pseudomonas is capsulated organism
Oxidation fermentation test..because itis strict aerobic..
Thank you Sagar Aryal sir..this is exactly the information I needed (infact this is the best information that i have found so far)..I too am a student of B.Sc. MB at St.xaviers ………extremely helpful information….thanks again
what is the difference between P.auroginosa and enteric bacteria