Biochemical Test and Identification of Enterococcus faecalis

Characteristics Enterococcus faecalis
Gram Staining Positive
Shape (Cocci/Diplococci/Rods) Cocci
Motility (Motile / Non-Motile) Non-Motile
Capsule (Capsulated/Non-Capsulated)
Spore (Sporing/Non-Sporing) Non-Sporing
Flagella (Flagellated/Non-Flagellated)
Catalase Negative (-ve)
Oxidase Negative (-ve)
VP Positive (+ve)
OF (Oxidative/Fermentative)
Indole Negative (-ve)
Citrate Negative (-ve)
Urease Negative (-ve)
Nitrate Reduction Positive (+ve)
H2S Negative (-ve)
PYR Positive (+ve)
Gelatin Hydrolysis Variable
Hemolysis (Alfa/Beta/Gamma) Variable (Alfa or Beta)
String Test
Pigment Negative (-ve)
Bile Solubility
Fermentation of
Arabinose Negative (-ve)
Fructose Positive (D-Fructose)
Glucose Positive (+ve)
Hippurate Positive (+ve)
Inulin Negative (-ve)
Lactose Positive (+ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (D-Mannose)
Raffinose Negative (D-Raffinose)
Ribose Positive (+ve)
Sorbitol Positive (+ve)
Sucrose Positive (+ve)
Enzymatic Reactions
Acetate Utilization
Acetoin Production
Acid Phosphatase
Alkaline Phosphatase Variable
Arginine dehydrolase Positive (+ve)
Beta Lactamase
Ornithine decarboxylase
Phenylalanine deaminase

15 thoughts on “Biochemical Test and Identification of Enterococcus faecalis”

  1. Which enterococci are typically indole positive and which are indole negative? The main two I would like to know is E. faecalis and E. faecium. Is there a permanency for any of them? E.g. Proteus mirabilis is always indole negative.

  2. How can Enterococcus faecalis can growth on macConky agar? While macC. is selective media for gram negative bacteria and inhibition gram positive bacteria?

  3. Could you please provide me with information of E. faecalis? I would need to know the culture conditions: humidity, temperture, culture medium, etc. and if this strain can be confused with other strains and the main reasons.

  4. Enterococci produce magenta red small colonies on MacConkey Agar, is tolerant to 6.5% NaCl, can grow at 42degree C, and Bile Esculin Positive, in Sodium Azide broth.

  5. E. faecalis also grows in the presence of bile and hydrolysis aesculin. We use a bike- aesculin plate for this purpose. If positive, the medium turns brown.

  6. Are you sure about the positive result for Nitrate reduction of E. faecalis? I’ve found some sources that say that E. faecalis is Nitrate reduction negative…

  7. I’m doing some research about E. faecalis and the VP gives me inconclusive, just as Mac Faddin’s book. I think it is one thing you should change.
    Anyway, your post it’s very helpful 🙂

  8. Results in Nitrate reduction can be inconclusive, but most of the time it leans to a positive result. The possibility of it being negative is probably because of malpractice or the nitrate compound was already reduced and the addition of Zinc may have reacted differently. If the tube turns red after the addition of the zinc, it means that unreduced nitrate was present. Therefore, a red color on the second step is a negative result. The addition of the zinc reduced the nitrate to nitrite, and the nitrite in the medium formed nitrous acid, which reacted with sulfanilic acid. The diazotized sulfanilic acid that was thereby produced reacted with the α-naphthylamine to create the red complex.

  9. Enterococcus faecalis is positive in Nitrate Reduction. It turned red after the addition of reagents A and B. All techniques and procedures done as laid out in the LAB MANUAL BIO 203 by MCDONLD in UCLA. MS Microbio student reporting. 🙂

  10. Really love it. I am a B.Sc Microbiology graduate and very passionate about Microbiology. I am currently seeking a masters degree. Please could you send me some Microbiology articles and latest information and researches.

  11. This was very helpful. E. faecalis also hydrolyzes esculin in the presence of bile, differentiating it from non-enterococcus group D strep.

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