| Characteristics | Salmonella Typhi |
|---|---|
| Capsule | Negative (-ve) |
| Catalase | Positive (+ve) |
| Citrate | Negative (-ve) |
| Flagella | Positive (+ve) |
| Gas | Negative (-ve) |
| Gelatin Hydrolysis | Negative (-ve) |
| Gram Staining | Negative (-ve) |
| Growth in KCN | Negative (-ve) |
| H2S | Positive (+ve) |
| Indole | Negative (-ve) |
| Motility | Motile |
| MR (Methyl Red) | Positive (+ve) |
| MUG Test | Negative (-ve) |
| Nitrate Reduction | Positive (+ve) |
| Oxidase | Negative (-ve) |
| Pigment | Negative (-ve) |
| Shape | Rod |
| Spore | Negative (-ve) |
| TSIA (Triple Sugar Iron Agar) | Alkali/Acid |
| Urease | Negative (-ve) |
| VP (Voges Proskauer) | Negative (-ve) |
| Fermentation of | |
| Adonitol | Negative (-ve) |
| Arabinose | Negative (-ve) |
| Arabitol | Negative (-ve) |
| Cellobiose | Negative (-ve) |
| DNase | Negative (-ve) |
| Dulcitol | Negative (-ve) |
| Erythritol | Negative (-ve) |
| Esculin Hydrolysis | Negative (-ve) |
| Glucose | Positive (+ve) |
| Glycerol | Negative (-ve) |
| Inositol | Negative (-ve) |
| Lactose | Negative (-ve) |
| Malonate | Negative (-ve) |
| Maltose | Positive (+ve) |
| Mannitol | Positive (+ve) |
| Mannose | Positive (+ve) |
| Melibiose | Positive (+ve) |
| Mucate | Negative (-ve) |
| MyoInositol | Negative (-ve) |
| Raffinose | Negative (-ve) |
| Rhamnose | Negative (-ve) |
| Salicin | Negative (-ve) |
| Sorbitol | Positive (+ve) |
| Sucrose | Negative (-ve) |
| Tartrate | Positive (+ve) |
| Trehalose | Positive (+ve) |
| Xylose | Positive (+ve) |
| Enzymatic Reactions | |
| Acetate Utilization | Negative (-ve) |
| Arginine Dehydrolase | Negative (-ve) |
| Esculin Hydrolysis | Negative (-ve) |
| Lipase | Negative (-ve) |
| Lysine | Positive (+ve) |
| ONPG (β-galactosidase) | Negative (-ve) |
| Ornithine Decarboxylase | Negative (-ve) |
| Peroxidase | Negative (-ve) |
| Tyrosine Hydrolysis | Negative (-ve) |
how can you tell the difference between Salmonella paratyphi b and salmonella choleraesuis? What biochemical results should be different between the 2?
hi, can you please tell me the difference between the result for the biochemical tests of S. typhi and S. typhimurium
first the sample should be inoculated to selective media/agar
Any biochemical test to differentiate Salmonella paratyphi B from S. typhimurium? Is there a test other than anti sera?
Salmonella paratyphi b will be citrate positive while salmonella typhi is negative.
Great research! please what is the difference between Salmonella Typhi,S.Paratyphi, s.enteritidis and s.typhimorium. Thanks
Biochemical tests
Presumptive colonies will be characterized using secondary biochemical tests:
On TSI slant: R/Y/H2S+/g+
Lysine decarboxylase: positive
Citrate utilization: positive
Urease: negative
Motility: positive
Indole: negative
MR: positive
VP: negative
After these tests you should subculture onto nutrient or tryptone soya agar
Sent for serotyping at Salmonella reference laboratory.
I have a problem with Salmonella spp.detection. I use your Anti- Salmonella I (A-E+Vi) serum. Colonies on specific media are specific to Salmonella, all biochemical test are also specific, but serum gives me a negative reaction.
nice practical research.and can you show me the basic difference&similarity between s.typhi and s.tyhpimurium ?????
Typhimurium is positive for citrate test typhi is negative. Other than that they are different in the diseases they produce with typhi producing typhoid fever and typhimurium most types of food poisoning from salmonella in poultry etc.
which antisera you used for salmonella typhi identification
nice performing but i am confusing that it is same for all Salmonella serovars….!
Are S.typhi citrate negative??? U have written negative.. but in Annanthanarayan it is written positive for all salmonella species. Could anyone tell me which one is correct?
It tested positive on citrate
All salmonella species are positive to citrate utilisation test, except salmonella typhi which is negative.
This depends on the test used. Simmons citrate agar is most commonly used and most of the host adapted serovars test negative (e.g., Dublin) because it is a minimal media and they are all auxitrophs that won’t grow on minimal media.
good research and clear guideline. you have given detail identification parameters for salmonella serovars especially salmonella typhi. I did it in the near past. particularly non-typhoidal serovars.
hi…kokeb which type of biochemical test you performed during your work on non typhiodal salmonella
I have performed the following tests:
1, TSI slant: R/Y/H2S+/g+
2, Lysine decarboxylase: positive
3, Citrate utilization: positive
4, Urease: negative
5, Motility: positive
6, Indole: negative
7, MR: positive
8, P: negative
Would this be Salmonella arizonae or Salmonella paratyphi B?