Biochemical Test and Identification of Salmonella Typhi


Salmonella Typhi

Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Negative (-ve)
Flagella Positive (+ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Negative (-ve)
H2S Positive (+ve)
Indole Negative (-ve)
Motility Motile
MR (Methyl Red) Positive (+ve)
MUG Test Negative (-ve)
Nitrate Reduction Positive (+ve)
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rod
Spore Negative (-ve)
TSIA (Triple Sugar Iron Agar) Alkali/Acid
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Arabitol Negative (-ve)
Cellobiose Negative (-ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Glucose Positive (+ve)
Glycerol Negative (-ve)
Inositol Negative (-ve)
Lactose Negative (-ve)
Malonate Negative (-ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Positive (+ve)
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Positive (+ve)
Sucrose Negative (-ve)
Tartrate Positive (+ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Acetate Utilization Negative (-ve)
Arginine Dehydrolase Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Lipase Negative (-ve)
Lysine Positive (+ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Peroxidase Negative (-ve)
Tyrosine Hydrolysis Negative (-ve)

11 thoughts on “Biochemical Test and Identification of Salmonella Typhi”

  1. Biochemical tests
    Presumptive colonies will be characterized using secondary biochemical tests:
     On TSI slant: R/Y/H2S+/g+
     Lysine decarboxylase: positive
     Citrate utilization: positive
     Urease: negative
     Motility: positive
     Indole: negative
     MR: positive
     VP: negative
    After these tests you should subculture onto nutrient or tryptone soya agar
    Sent for serotyping at Salmonella reference laboratory.

  2. I have a problem with Salmonella spp.detection. I use your Anti- Salmonella I (A-E+Vi) serum. Colonies on specific media are specific to Salmonella, all biochemical test are also specific, but serum gives me a negative reaction.

  3. nice practical research.and can you show me the basic difference&similarity between s.typhi and s.tyhpimurium ?????

    • Typhimurium is positive for citrate test typhi is negative. Other than that they are different in the diseases they produce with typhi producing typhoid fever and typhimurium most types of food poisoning from salmonella in poultry etc.

  4. Are S.typhi citrate negative??? U have written negative.. but in Annanthanarayan it is written positive for all salmonella species. Could anyone tell me which one is correct?

  5. good research and clear guideline. you have given detail identification parameters for salmonella serovars especially salmonella typhi. I did it in the near past. particularly non-typhoidal serovars.

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