…mechanism depending on the antibiotic used. Example: aminoglycosides 4. Classification on basis of chemical composition β lactam antibiotics: penicillin, cephalosporin, carbapenem, monbactam Tetracycline group of antibiotics Aminoglycosides: streptomycin, neomycin, kanamycin,…
…are examples). Heterophile antigens are identical antigens found in the cells of different species. Examples: Forrssman antigen, Cross-reacting microbial antigens, etc. Adjuvants are substances that are non-immunogenic alone but enhance…
…majority of contaminants surviving decontamination of the specimen. This formulation also encourages the earliest possible growth of mycobacteria. Composition of LJ Medium IngredientsAmountPotato Flour (Potato Starch) 30.0 gm L-Asparagine 3.6 gm…
…for the selective isolation of pathogenic Vibrio species. Composition of TCBS Agar Ingredients per liter of deionized water:* IngredientsSucrose 20.0 gm Dipeptone 10.0 gm Sodium Citrate 10.0 gm Sodium Thiosulfate 10.0…
…Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. Cappuccino J.G. and Sherman N. 2008. Microbiology: A Laboratory Manual, 8th ed. Pearson Benjamin Cummings, San Francisco, CA, USA. vlab.amrita.edu/?sub=3&brch=73&sim=697&cnt=1 www.asmscience.org/content/education/protocol/protocol.3658 https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/MotilityTestMedia.htm…
…at least 4 weeks before being discarded. Molecular detection The use of molecular techniques facilitates the identification of Alternaria using the Basic Local Alignment Search Tool (BLAST) which allows comparing…
…St. Louis, Missouri: Elsevier. Cappuccino J.G. and Sherman N. 2008. Microbiology: A Laboratory Manual, 8th ed. Pearson Benjamin Cummings, San Francisco, CA, USA. www.vumicro.com/vumie/help/VUMICRO/Hydrogen_Sulfide_Production_Test.htm mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-8-3-397 https://microbenotes.com/hydrogen-sulfide-h2s-production-test/ www.microbiologyresearch.org/docserver/fulltext/micro/8/3/mic-8-3-397.pdf?expires=1543332392&id=id&accname=guest&checksum=C42277C712F05E5951241893FC8EC3F7 spot.pcc.edu/~jvolpe/b/bi234/lab/differentialMedia/H2Sproduction.html https://senthilprabhu.blogspot.com/2017/10/hydrogen-sulphide-h2s-production-test.html https://jb.asm.org/content/jb/10/5/439.full.pdf…
…piece or T piece. S-IgA is secretory component (SC) is an additional component of IgA which is a polypeptide of 75- 80 kDa comprising the extracellular proteolytic fragment of the…
…accuracy of interpretation, uninoculated control tubes and/or inoculated Phenol Red Broth Base control tubes should be run in parallel with the fermentation tests. Reference https://homepages.wmich.edu/~rossbach/bios312/LabProcedures/Biochem%20characteristics%20procedures.html www.himedialabs.com/TD/M054.pdf www.austincc.edu/microbugz/phenol_red_broth.php https://quizlet.com/56724336/phenol-red-broth-flash-cards/ foodsafety.neogen.com/pdf/acumedia_pi/7148_pi.pdf spot.pcc.edu/~jvolpe/b/bi234/lab/differentialMedia/CarboFermentationTest2.html…
…proteins, sugar, etc., in the blood. Plasma aids in the maintenance of blood pressure and in the regulation of body temperature References https://medimoon.com/2012/07/difference-between-plasma-and-serum/ https://www.reference.com/science/difference-between-plasma-serum-58673501a3b3e1af https://www.quora.com/What-is-difference-between-plasma-and-serum https://www.protocol-online.org/biology-forums/posts/4959.html https://www.slideshare.net/nahomephrem/nahom-ppt https://health.onehowto.com/article/how-you-can-tell-the-difference-between-serum-and-plasma-1847.html https://www.differencebetween.com/difference-between-plasma-and-vs-serum/ http://www.gembio.com/post/what-difference-between-clot-and-plasma-derived-human-serum…
…infection to health care workers, laboratory personnel, and others. Examples of specific precautions include the following: Gloves should be used when handling all potentially infectious materials; protective garments should be…
…Dextrose Agar with Chloramphenicol is recommended for the selective cultivation of fungi from mixed samples. Composition of Potato Dextrose Agar (PDA) Preparing Ourself CompositionPotato infusion 200 gm Dextrose 20 gm…
…for preservation and storage of bacterial cultures · Crystal violet blood agar Selective media for Streptococcus pyogenes · Cysteine Lactose Electrolyte De?cient Media (CLED Media) Most commonly used media for…
…a modified or derived thrombin molecule, to from a coagulase-CRP complex. This complex in turn reacts with fibrinogen to produce the fibrin clot. Procedure and Types of Coagulase Test Slide…
…is to unite investigators conducting microbiome research in environmental, agricultural, and biomedical arenas. Topics broadly addressing the study of microbial communities, such as, microbial surveys, bioinformatics, meta-omics approaches and community/host…
…be healthy and free from any communicable diseases. Suckling mice(less than 48 hours old) are most commonly used. Suckling mice are susceptible to togavirus and coxsackie virues, which are inoculated…
…2014. Bailey and Scott’s diagnostic microbiology. Thirteen edition. Mosby, Inc., an affiliate of Elsevier Inc. 3251 Riverport Lane. St. Louis. Missouri 63043 https://www.austincc.edu/microbugz/starch_hydrolysis.php https://www.himedialabs.com/TD/M107.pdf https://www.uwyo.edu/molb2210_lab/info/biochemical_tests.htm https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/StarchAgar.html https://www.yourarticlelibrary.com/experiments/starch-hydrolysis-test-on-bacteria-to-find-out-their-ability-to-hydrolyse-starch/26587 https://www.sas.upenn.edu/LabManuals/biol275/Table_of_Contents_files/21-DiagnosticTests.pdf http://www.asmscience.org/content/education/imagegallery/image.3172 https://biocyclopedia.com/index/biotechnology_methods/microbiology/starch_hydrolysis_test_ii_method.php…
…in complement fixing as compared to IgG1. IgG2 is especially important as a repository for antibodies against antigens from many common bacteria that cause pneumonia. A deficiency in IgG2 is…
…B.D. Skerman, A guide to the identification of the genera of bacteria, The Williams & Wilkins Co., Baltimore, MD, p.218 – 220 (1967) https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma-Aldrich/Datasheet/1/73426dat.pdf www.biologypractical.com/nitrate-reduction-test-objective-principle-procedure-and-result/ delrio.dcccd.edu/jreynolds/microbiology/2420/files/nitrate.pdf https://microbenotes.com/nitrate-reduction-test-objectives-principle-procedure-and-results/ www.austincc.edu/microbugz/nitrate_reduction.php www.asmscience.org/content/education/protocol/protocol.3660 https://catalog.hardydiagnostics.com/cp_prod/content/hugo/nitratereagent.htm…
…pus are stained by Giemsa or Wright’s stains and examined microscopically. Skin scrapings, exudates and body fluids should be examined using 10% KOH and Parker ink or calcofluor white mounts….
Welcome to MicrobiologyInfo.com, constantly growing and evolving collection of microbiology notes and information. Whether you’re a student, professor, working in the medical field or just curious about microbiology, I’m sure…
…Gunn B. A. (1976). SXT and Taxo A disks for presumptive identification of group A and B streptococci in throat cultures. Journal of clinical microbiology, 4(2), 192-3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5730933/ https://www.labce.com/spg15135_group_a_strep_a_disksxt.aspx https://www.coursehero.com/file/p3ess2oj/SXT-disc-susceptibility-test-used-for-the-presumptive-identification-of-beta/ https://www.dalynn.com/dyn/ck_assets/files/tech/DB10.pdf https://www.researchgate.net/publication/232229031_Is_Streptococcus_pyogenes_Resistant_or_Susceptible_to_Trimethoprim-Sulfamethoxazole…
…that possess the enzyme lipase hydrolyze the free fats present in the medium to form glycerol and free fatty acids. Consequently, the release of insoluble free fatty acids results in…
…exhibit a wide zone (2-4 mm wide). Beta hemolysis is more marked when the plate has been incubated anaerobically. They are generally commensals of throat and causes opportunistic infections. Examples: Streptococcus pyogenes,…
…the urinary system. Crystals can continue to form after micturition. Crystal precipitation after micturition is most commonly due to changes in temperature, as can occur if the urine is stored at room temperature or…
…comprising of basic heavy and light-chain antibody structure but exists in pentameric (secretory) and monomeric configurations. The IgM polymer is composed of subunits in which two heavy chains (µ) are…
…confirmed as lactobacilli by appropriate biochemical testing. References Tille, P. M., & Forbes, B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/LactobacilliMRSBroth.html www.labm.com/products/m-r-s-broth.asp https://foodsafety.neogen.com/pdf/acumedia_pi/7406_pi.pdf www.scharlabmagyarorszag.hu/katalogus/02-135_TDS_EN.pdf https://www.bd.com/europe/regulatory/Assets/IFU/Difco_BBL/288110.pdf…
Introduction of Flagella Flagella are the complex filamentous cytoplasmic structure protruding through cell wall. These are unbranched, long, thread like structures, mostly composed of the protein flagellin, intricately embedded in…
…Standardized API strips have a long shelf life, enabling every laboratory to keep the test kits on hand. Allow accurate identifications based on extensive databases References https://www.biomerieux-usa.com/clinical/api https://bio.libretexts.org/Demos%2C_Techniques%2C_and_Experiments/Microbiology_Labs_I/43%3A_API-20E_multitest_strip https://www.jlindquist.com/generalmicro/102bactid2.html https://jcm.asm.org/content/jcm/7/6/539.full.pdf…
…P.M. 2014. Bailey and Scott’s diagnostic microbiology. Thirteen edition. Mosby, Inc., an affiliate of Elsevier Inc. 3251 Riverport Lane. St. Louis. Missouri 63043 http://iws2.collin.edu/dcain/CCCCD%20Micro/sim_deep.htm https://www.austincc.edu/microbugz/sim_medium.php https://spot.pcc.edu/~jvolpe/b/bi234/lab/differentialTests/SIM%20Medium.html http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0435&c=UK&lang=EN https://www.himedialabs.com/TD/M181.pdf https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/SIMMedium.htm https://www.coursehero.com/file/p3orm5tu/Hydrogen-Sulfide-Production-and-Motility-SIM-Medium-is-used-to-test-a-microbe/…