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…Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., American Public Health Association, Washington, D.C. himedialabs.com/TD/M078.pdf https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/KliglerIronAgarKIA.htm foodsafety.neogen.com/pdf/acumedia_pi/7140_pi.pdf https://assets.thermofisher.com/TFS-Assets/LSG/manuals/IFU453621.pdf https://mltgeeks.com/kligler-iron-agar-principlescomposition-and-slants-interpretation/…

…nutrient carryover. References Tille P.M. 2014. Bailey and Scott’s diagnostic microbiology. Thirteen edition. Mosby, Inc., an affiliate of Elsevier Inc. 3251 Riverport Lane. St. Louis. Missouri 63043 https://catalog.hardydiagnostics.com/cp_prod/content/hugo/XVFactorDisks.htm http://www.apsi.it/public/ufiles/smi/tp38_3_en_150108.pdf https://himedialabs.com/TD/DD022.pdf…

…agar doesnot rule out an identification of Pseudomonas aeruginosa. References Tille, P. M., & Forbes, B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. https://microbiologyinfo.com/cetrimide-agar-composition-principle-uses-preparation-and-colony-morphology/ www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0579&c=UK&lang=EN https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/CetrimideSelAgar.htm himedialabs.com/TD/M024B.pdf…

…donor compounds (NADH) to electron acceptors (usually oxygen).  The test reagent, N, N, N’, N’-tetramethyl-p-phenylenediamine dihydrochloride acts as an artificial electron acceptor for the enzyme oxidase. The oxidised reagent forms…

…the tube prior to inoculation if bubbles are trapped in the durham tube. Trapped bubbles that are not released may lead to false-positive results. Reference https://catalog.hardydiagnostics.com/cp_prod/content/hugo/purplebrothdt.htm www.bd.com/europe/regulatory/Assets/IFU/Difco_BBL/222810.pdf https://www.himedialabs.com/TD/M284.pdf tools.thermofisher.com/content/sfs/manuals/IFU454351.pdf https://us.vwr.com/store/asset?assetURI=https://us.vwr.com/stibo/hi_res/std.lang.all/18/44/8041844.pdf…

…glucose only and produce a yellow color in the medium. A slight bluing (blue-green) of the medium may occur after prolonged incubation. References https://catalog.hardydiagnostics.com/cp_prod/content/hugo/malonatebroth.htm www.vumicro.com/vumie/help/VUMICRO/Malonate_utilization_Test.htm www.bd.com/europe/regulatory/Assets/IFU/Difco_BBL/239520.pdf https://assets.thermofisher.com/TFS-Assets/LSG/manuals/IFU61324.pdf atlas.sund.ku.dk/microatlas/veterinary/pheno_tests/Malonate_Test/ www.interlabdist.com.br/dados/produtos/bula/doc/1713148ff81d57aae4.pdf1 https://himedialabs.com/TD/M382.pdf…

…or suppressed with organisms other than Citrobacter spp. References Tille, P. M., & Forbes, B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. himedialabs.com/TD/M377.pdf www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0381&c=UK&lang=EN iws2.collin.edu/dcain/CCCCD%20Micro/lysine_iron_agar.htm https://assets.thermofisher.com/TFS-Assets/MBD/Instructions/IFU453772.pdf foodsafety.neogen.com/pdf/acumedia_pi/7211_pi.pdf…

…ed. Pearson Benjamin Cummings, San Francisco, CA, USA. Brown A.E. 2009.  Benson’s Microbiological Applications: Laboratory Manual in General Microbiology, 11th ed. McGraw-Hill Companies, New York, NY, USA. delrio.dcccd.edu/jreynolds/microbiology/2421/lab_manual/lipid.pdf https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma-Aldrich/Datasheet/1/91015dat.pdf www.srlchem.com/products/product_details/productId/3716/Tributyrin-Agar-Base-w-o-Tributyrin…

…diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. Cappuccino J.G. and Sherman N.  2008.  Microbiology: A Laboratory Manual, 8th ed. Pearson Benjamin Cummings, San Francisco, CA, USA. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/EggYolkAgarModified.html www.vetbact.org/popup/popup.php?id=34&LANG=en https://www.ncbi.nlm.nih.gov/pmc/articles/PMC291196/?page=1 vlab.amrita.edu/?sub=3&brch=73&sim=974&cnt=1…

…B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA. https://catalog.hardydiagnostics.com/cp_prod/content/hugo/pyruvatebroth.htm https://www.dalynn.com/dyn/ck_assets/files/tech/TP99.pdf https://jb.asm.org/content/jb/88/1/4.full.pdf http://www.quelab.com/htmleng/2231a.html…

…while it is warm may result in false-negative interpretation. References Tille, P. M., & Forbes, B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. www.asmscience.org/content/education/protocol/protocol.3776 www.austincc.edu/microbugz/gelatinase_test.php www.biologypractical.com/gelatin-hydrolysis-test-principle-procedure/…

…result. References Tille P.M. 2014. Bailey and Scott’s diagnostic microbiology. Thirteen edition. Mosby, Inc., an affiliate of Elsevier Inc. 3251 Riverport Lane. St. Louis. Missouri 63043 https://www.dalynn.com/dyn/ck_assets/files/tech/TS27.pdf https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/NaCl65Media.htm https://www.austincc.edu/microbugz/handouts/Salt%20Tolerance.pdf https://www.bioscience.com.pk/topics/microbiology/item/239-salt-tolerance-test…

…is an example of a commercially available chromogenic test (BD Microbiology Systems, Cockeysville, Maryland). The disk incorporates nitrocefin as the substrate. It exhibits a very rapid color change from yellow…

…microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. Cappuccino J.G. and Sherman N.  2008.  Microbiology: A Laboratory Manual, 8th ed. Pearson Benjamin Cummings, San Francisco, CA, USA. spot.pcc.edu/~jvolpe/b/bi234/lab/differentialMedia/DNaseTest.htm https://www.austincc.edu/microbugz/dnase_test.php https://www.bd.com/europe/regulatory/Assets/IFU/HB/CE/PA/PA-255506.pdf https://himedialabs.com/TD/M482.pdf https://www.bioscience.com.pk/topics/microbiology/item/230-dnase-test…

…B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA. https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/CRITN-PhenylalanineAgar.html himedialabs.com/TD/M281.pdf www.austincc.edu/microbugz/phenylalanine_deaminase_test.php https://assets.fishersci.com/TFS-Assets/MBD/Instructions/IFU62180.pdf…