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Cetrimide Test – Principle, Procedure, Uses and Interpretation

…agar doesnot rule out an identification of Pseudomonas aeruginosa. References Tille, P. M., & Forbes, B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. https://microbiologyinfo.com/cetrimide-agar-composition-principle-uses-preparation-and-colony-morphology/ www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0579&c=UK&lang=EN https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/CetrimideSelAgar.htm himedialabs.com/TD/M024B.pdf…

Sabouraud Dextrose Agar (SDA) – Composition, Principle, Uses, Preparation and Colony Morphology

…from material containing large numbers of other fungi or bacteria. This medium is also employed to determine microbial contamination in food, cosmetics, and clinical specimens. Preparation of SDA Combine all ingredients in…

Germ Tube Test- Principle, Procedure, Results, Interpretation and Limitations

…test. So “differential tube test” or another test name could be more perfect/suitable for use. Replay would be very helpfull, thanks. Sandy What happens when you exceed on incubation time…

Salt Tolerance Test – Principle, Procedure, Uses and Interpretation

…result. References Tille P.M. 2014. Bailey and Scott’s diagnostic microbiology. Thirteen edition. Mosby, Inc., an affiliate of Elsevier Inc. 3251 Riverport Lane. St. Louis. Missouri 63043 https://www.dalynn.com/dyn/ck_assets/files/tech/TS27.pdf https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/NaCl65Media.htm https://www.austincc.edu/microbugz/handouts/Salt%20Tolerance.pdf https://www.bioscience.com.pk/topics/microbiology/item/239-salt-tolerance-test…

Gelatin Hydrolysis Test – Principle, Procedure, Uses and Interpretation

…while it is warm may result in false-negative interpretation. References Tille, P. M., & Forbes, B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. www.asmscience.org/content/education/protocol/protocol.3776 www.austincc.edu/microbugz/gelatinase_test.php www.biologypractical.com/gelatin-hydrolysis-test-principle-procedure/…

Immunoglobulin A (IgA) – Structure, Properties, Subclasses, Functions

…piece or T piece. S-IgA is secretory component (SC) is an additional component of IgA which is a polypeptide of 75- 80 kDa comprising the extracellular proteolytic fragment of the…

Butyrate Disk Test – Principle, Procedure, Uses and Interpretation

…as staphylococci and pseudomonads may also give positive results. References Tille, P. M., & Forbes, B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. https://www.thermofisher.com/order/catalog/product/R21121 https://catalog.hardydiagnostics.com/cp_prod/content/hugo/CatScreen.htm https://www.keyscientific.com/BUTYRATE-DISCS-differentiate-Branhamella-Catarrhalis-from-Neisseria-spp.-by-demonstrating-the-enzymatic-hydrolysis-of-bromo-chloro-indolyl-butyrate.-50-discs.html…

Leucine amino peptidase (LAP) Test – Procedure, Uses and Interpretation

…LAP test. Adequate inoculums should be ensured otherwise false negatives may occur Streptococci should be tested prior to 48-hour incubation or subcultured prior to testing. References www.dalynn.com/dyn/ck_assets/files/tech/DL10.pdf https://assets.thermofisher.com/TFS-Assets/LSG/manuals/IFU21129.pdf https://eportal.mountsinai.ca/Microbiology/manual/tech/tech23.pdf https://www.sigmaaldrich.com/catalog/product/sial/75554?lang=en&region=NP…

Pyruvate Broth Test – Principle, Procedure, Uses and Interpretation

…B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA. https://catalog.hardydiagnostics.com/cp_prod/content/hugo/pyruvatebroth.htm https://www.dalynn.com/dyn/ck_assets/files/tech/TP99.pdf https://jb.asm.org/content/jb/88/1/4.full.pdf http://www.quelab.com/htmleng/2231a.html…

Citrate Utilization Test- Principle, Media, Procedure and Result

Result Interpretation of Citrate Utilization Test

…not necessarily as the sole carbon source. Media used in Citrate Utilization Test Simmon’s Citrate Agar Composition CompositionSodium Chloride 5.0 gm Sodium Citrate (dehydrate) 2.0 gm Ammonium Dihydrogen Phosphate 1.0…

CAMP Test- Principle, Uses, Procedure and Result Interpretation

…causative agents of mastitis in cows. CAMP is an acronym for the authors of this test (Christie, Atkinson, Munch, and Peterson) which was identified in 1944. Principle of CAMP test…

Starch Casein Agar (SCA) – Composition, Principle, Uses, Preparation and Result Interpretation

Actinomycetes on Starch Casein Agar (SCA)

…environment and plays significant role not only in therapeutic applications but also on recycling of organic matter. Composition of Starch Casein Agar IngredientsGms / LitreSoluble starch 10.00 Casein (Vitamin Free)…

Hepatitis B Virus- Structure, Epidemiology, Symptoms, Pathogenesis, Diagnosis, Treatment and Vaccines

…strong detergent. HBcAg, hepatitis B core antigen. Contains icosahedral nucleocapsid, the core (27 nm) and outer envelope (4 nm) There is an outer shell (or envelope) composed of lipid and…

List of culture media used in microbiology with their uses

…for preparation for carbohydrate fermentation media · To ascertain whether a bacteria is motile or non-motile · Basis for Indole test · Phenol-Red Egg Yolk Polymyxin Agar Selective media for…

X and V factor Test – Principle, Procedure, Uses and Interpretation

…nutrient carryover. References Tille P.M. 2014. Bailey and Scott’s diagnostic microbiology. Thirteen edition. Mosby, Inc., an affiliate of Elsevier Inc. 3251 Riverport Lane. St. Louis. Missouri 63043 https://catalog.hardydiagnostics.com/cp_prod/content/hugo/XVFactorDisks.htm http://www.apsi.it/public/ufiles/smi/tp38_3_en_150108.pdf https://himedialabs.com/TD/DD022.pdf…

Lowenstein-Jensen (LJ) Medium- Composition, Principle, Uses, Preparation and Colony Morphology

…600 ml of distilled water containing 12 ml of glycerol. Heat if necessary to dissolve the medium completely. Autoclave at 121°C for 15 minutes. Prepare 1000 ml of a uniform…

Selenite F Broth- Composition, Principle, Uses, Preparation and Result Interpretation

…the isolation of Salmonella and Shigella from faecal specimens. Composition of Selenite F Broth CompositionCasein enzymic hydrolysate 5 gm/L Lactose 4 gm/L Sodium phosphate 10 gm/L Sodium hydrogen selenite (NaHSeO3)…

Simmons Citrate Agar- Composition, Principle, Uses, Preparation and Result Interpretation

Result Interpretation on Simmons Citrate Agar

…growth on citrate and do not produce a color change. Growth is considered a positive citrate utilization test, even in the absence of a color change. Tests with equivocal results…

Online Microbiology Notes – MicrobiologyInfo.com

Microbiology Info Frontpage Featured Image

…Shape and Arrangement of Bacterial Cells All About Benedict’s Test Best Microbiology Books Differences Between Exotoxins and Endotoxins About the Author – Sagar Aryal My name is Sagar Aryal and…

The Triple Sugar Iron (TSI) Test – Principle, Procedure, Uses and Interpretation

…test is a microbiological test named for its ability to test a microorganism’s ability to ferment sugars and to produce hydrogen sulfide. An agar slant of a special medium with…

Immunoglobulin G (IgG) – Properties, Structure and Functions

…in complement fixing as compared to IgG1. IgG2 is especially important as a repository for antibodies against antigens from many common bacteria that cause pneumonia. A deficiency in IgG2 is…

Lecithinase Test – Principle, Procedure, Uses and Interpretation

…is recommended that biochemical, immunological, molecular, or mass spectrometry testing be performed on colonies from pure culture for complete identification. Maintenance of anaerobic condition is compulsory. A negative lecithinase test…

MacConkey Agar- Composition, Principle, Uses, Preparation and Colony Morphology

…their composition, uses, preparation and their appearances /colour after culturing. yara how come staph is pale pink while it dose’t grow on macc at all ? Rajbir Kaur Is it…

Cetrimide Agar- Composition, Principle, Uses, Preparation and Colony Morphology

…encountered. The testing is for information only so we have no major concerns, but we don’t want to be performing a test if it is of no value at all….

Amino Acid Decarboxylase Test – Procedure, Uses and Interpretation

…brought about by a positive decarboxylation reaction. References Tille, P. M., & Forbes, B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. https://www.microrao.com/micronotes/pg/amino_acids.pdf delrio.dcccd.edu/jreynolds/microbiology/2421/lab_manual/decarb_deam.pdf https://microbeonline.com/decarboxylation-test-types-uses-principles-procedure-results/ himedialabs.com/TD/M912S.pdf static.lvengine.net/frilabo/Imgs/outros/88014.TechnicalDataSheet.pdf…

Biochemical Test and Identification of Enterococcus faecalis

…B.Sc Microbiology graduate and very passionate about Microbiology. I am currently seeking a masters degree. Please could you send me some Microbiology articles and latest information and researches. Andrew Enterococcus…

Motility Test – Principle, Procedure, Uses and Interpretation

…to assign taxonomic classification to organisms. Motility tests are important in characterization of pathogens. The tests are often employed in identification protocols in the family Enterobacteriaceae Motility test is also used…

Hippurate Hydrolysis Test – Procedure, Uses and Interpretation

…for complete identification. Insufficient inoculum may result in erroneous results. References Tille, P. M., & Forbes, B. A. (2014). Bailey & Scott’s diagnostic microbiology (Thirteenth edition.). St. Louis, Missouri: Elsevier. https://catalog.hardydiagnostics.com/cp_prod/content/hugo/HippurateTest.htm himedialabs.com/td/dd035.pdf…

String test for the identification of Vibrio cholerae

…Vibrio cholerae (string test positive) from other Vibrio spp. (string test negative). Procedure of String Test Take a clean grease free slide and add a drop of 0.5% sodium deoxycholate or Sodium…

Phenol Red Fermentation Test – Principle, Procedure, Uses and Interpretation

…accuracy of interpretation, uninoculated control tubes and/or inoculated Phenol Red Broth Base control tubes should be run in parallel with the fermentation tests. Reference https://homepages.wmich.edu/~rossbach/bios312/LabProcedures/Biochem%20characteristics%20procedures.html www.himedialabs.com/TD/M054.pdf www.austincc.edu/microbugz/phenol_red_broth.php https://quizlet.com/56724336/phenol-red-broth-flash-cards/ foodsafety.neogen.com/pdf/acumedia_pi/7148_pi.pdf spot.pcc.edu/~jvolpe/b/bi234/lab/differentialMedia/CarboFermentationTest2.html…