…a zone of proteolysis, which is demonstrated by a clear area surrounding the bacterial growth. This loss of opacity is the result of a hydrolytic reaction yielding soluble, non-colloidal amino…
Cetrimide is a quaternary ammonium salt, which acts as a selective agent and inhibits most bacteria by acting as a cationic detergent (Cetyltrimethylammonium bromide). Cetrimide when added into a culture…
Deoxyribonucleic acid (DNA) is a large polymer of nucleotides that is way too large to enter the cell membrane. In order to utilize external DNA, bacteria cells secrete exoenzymes (DNases)…
The ability of bacteria to form organic compounds by metabolizing certain carbohydrates and related compounds is a widely used method for the identification of microorganisms. Different fermentation media are used…
…phospholipase C, which is an enzyme that splits the phospholipid lecithin. Such lecithinase activity is used to characterize several gram positive and gram negative bacteria. Objectives To determine the ability…
…and repackaging of DNA during DNA replication and the bacterial cell cycle. Novobiocin binds to DNA gyrase, and blocks adenosine triphosphatase (ATPase) activity. Susceptibility to novobiocin is determined by placing…
…fermentation of carbohydrates by bacteria. Principle Whether an organism is oxidative or fermentative can be determined by using Hugh and Leifson’s medium, commonly called as OF medium which contain tryptone…
The antigen used to define serological test of Salmonella include Somatic O antigen, Flagellar H antigen and Vi antigen (Capsular antigen). Some of the differences between O Antigen and H…
kenesa what is the difference between P.auroginosa and enteric bacteria Amit Khanal Thank you Sagar Aryal sir..this is exactly the information I needed (infact this is the best information that…
…sound results in a report. It is also quite difficult to clean any glassware after that mistake. Bamidele Love What is the differences between Benedict solution and Fehling’s solution Rin…
Ahmed Hello. Very good effort however in differences between serum and plasma you stated in No. 8 that plasma doesn’t contain antibodies. This is not true as you know because…
…in excess. Degradation of xylose, lactose and sucrose to acid causes phenol red indicator to change its color to yellow. Bacteria that decarboxylate lysine to cadaverine can be recognized by the appearance…
Vibrio cholerae is a gram-negative bacilli bacteria with a “comma” shaped body and a flagella for the movement. V. cholerae and V. mimicus are the only Vibrio spp. that do…
…presence of an intracellular autolytic enzyme, amidase. Bile salts lower the surface tension between the bacterial cell membrane and the medium, thus accelerating the organism’s natural autolytic process. Bile salts…
…Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarbrucken, Germany. He is interested in research related to actinobacteria, myxobacteria, and natural products. Sagar has published more than a dozen papers in reputed…
Hippurate hydrolysis test is used to detect the ability of bacteria to hydrolyse substrate hippurate into glycine and benzoic acid by action of hippuricase enzyme present in bacteria. Hippuricase is…
Acetamide test is used to test an organism’s ability to utilize acetamide by deamidation. It is used in the qualitative procedures to differentiate non-fermentative, gram negative bacteria, especially Pseudomonas aeruginosa,…
Acetate utilization test is employed in the qualitative procedures to differentiate species of Shigella from Escherichia coli and non-fermentative gram negative bacteria. Objective To differentiate organisms based on ability to…
…(susceptible). Principle The antibiotic bacitracin inhibits the synthesis of bacterial cell walls by interfering the peptidoglycan synthesis of bacteria. A disk impregnated with a small amount of bacitracin (0.04 units) is placed…
…Gelatinases are proteases secreted extracellularly by some bacteria which hydrolyze or digest gelatin. The production of gelatinases is used as a presumptive test for the identification of various organisms, including…
…be used for this determination, depending on the type of organism being tested. Objectives To determine the ability of microorganisms to produce the enzyme lipase. To identify the bacteria on…
…or purplish blue coloration on the disc after the introduction of bacterial colony on the disc. All micrococci possess cytochrome c, whereas most staphylococci, with few exceptions, lack this type…
…of lactobacilli in general. Hence, MRS agar and broth given to their superiority are commonly employed for culturing and identifying of Lactic acid bacteria especially the Lactobacillus spp. “Lactic acid…
…or blackening of the line of inoculation indicates the presence of bacteria producing hydrogen sulfide. Negative: A negative H2S test is denoted by the absence of blackening. Uses It is…
…It disrupts the synthesis of purines, thymidine, and methionine which are needed for the production of DNA and proteins during bacterial replication. Thus the net effect of each of these drugs…
…Sphingomyelinase activity on RBCs (responsible for CAMP test) – Displays hot-cold phenomenon δ-Hemolysin – Surfactant on various cells: erythrocytes, leucocytes, bacterial protoplasts γ-Hemolysin – γ-Hemolysins composed of two proteins Leukocidin…
…macConky agar? While macC. is selective media for gram negative bacteria and inhibition gram positive bacteria? Richard I wonder !!!! The bile salt and Crystal violet was inactive or what…
…Selective media containing aminoglycosides or 1:500,000 crystal violet selectively permit the growth of Streptococci in a mixed culture and inhibit other bacteria. 7. Colony on blood agar is non-pigmented. 8….
…growth of H. influenzae. influenzae will show Satellitism. V-Factor is involved in oxidation-reduction process in the growing bacterial cell. influenzae requires pantothenic acid, thiamine and uracil. Biochemical Tests TestReactionCatalase +…
Microbiology is the study of microorganisms like bacteria, virus, fungi, protozoans which is seen only under the microscope and which have many economic importance. These are the world top 50…